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Rede Proteómica Portuguesa
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ProCura- Research Institution MembersLaboratório de Proteómica - CGH/INSA-Ricardo Jorge - LisbonGroup of Hemoglobinopaties (Hb) - CGH/INSA-Ricardo Jorge - LisbonBioinformatic Unit - IGCInstituto de Biologia Molecular e Celular do Porto (IBMC) - Departament of Molecular NeurobiologyMass Spectometry Laboratory - ITQB/IBETMolecular Biology Laboratory - Institute of Hygiene and Tropical MedicineCentro de Malária e outras Doenças Tropicais, U.E.I. Malária, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa – Host-parasite interactions
ProCura-University Members Departament of Chemistry, University of AveiroREQUIMTE Laboratório Associado, CQFB, Departamento de Química (FCT/UNL)
ProCura- Research Institution Members Laboratório
de Proteómica
Missions - Developing an innovative proteomics technical
platform to the diagnosis, prognosis and assessment of infectious
and degenerative chronic diseases.
2005-2006 Proteomics of Chronic Lung Diseases:
Leading to Biomarkers and Therapeutic Target Discovery. 2006-2007 Is the MicroRNA-122 specific determinant
for HCV virus replication in the chronic liver hepatitis? 2007- 2008 Autoimmunity in Behçet's
Disease - a Proteomics Approach.
Deborah
Penque, Reseacher Lab Coordinator Mª Fátima Vaz, Principal Assistant of Technical Healthy Career Lab Technical Responsible Tânia Simões, Post-Doctoral Fellow (FCT* ) Isabel Carvalho-Oliveira, Doctoral Fellow (FCT*) Patrícia Gomes Alves, Doctoral Fellow (FCT*) Bruno M. Alexandre, Doctoral Fellow (FCT*) Nuno Miguel Charro, Doctoral Fellow (FCT*) Judite Marques Dias, Doctoral Fellow (FCT*) João Banha, Research Fellow INSA Sofia Neves, Research Fellow APEF** *Fundação da Ciência e a Tecnologia; **Associação Portuguesa para o Estudo do Fígado
For
the last ten years, this group has been actively involved in studying
the molecular basis of alfa- and ß-thalassemia in the Portuguese
population, by investigating a number of genetic alterations associated
with hemoglobin disorders, both at the genomic and functional levels.
Presently, our studies are specifically focused on the role of mRNA
stability in the control of human globin gene expression. In this
context, we are developing the following projects:
The
operating team of this Unit is responsible for the Portuguese node
of the EMBnet for the last ten years, delivering to many users a
service that consists of providing access to periodically updated
copies of about 30 databases of sequence data, a suite of correctly
installed and documented programs to process them and user support
via e-mail or telephone. Specific bridging competence in the genomics/proteomics
area is presently one of the major targets of the current investments
in both tools and human expertise. The ProCura network is expected
to bring in some of the most challenging opportunities to field
test and deploy such facilities in full contact with the user community. Instituto de Biologia Molecular e Celular do Porto (IBMC) - Departamento de Neurobiologia Molecular
Molecular Neurobiology Group Leader: Maria João Saraiva , PhD (Biomedical Inst. U.P.) Principal Investigators: This unit had its origins in 1997 with a background
on studies on familial amyloidotic polyneuropathy (FAP), a peculiar
form of neuropathy first described by the eminent Portuguese neurologist
Corino de Andrade, which is characterized by the deposition of amyloid
in special on the peripheral nerves. This autosomal dominant disease
is a health problem in the country. The unit is devoted to laboratorial
investigation on FAP on epidemiological, biochemical, genetic and
pathological aspects, as well as on the biology of transthyretin-
TTR (represented in the figure), a serum protein that when mutated
is the major defect underlying FAP.
ITQB/IBET - Mass Spectometry Laboratory
Coordinator: Ana Coelho, PhD (labms@itqb.unl.pt) The
ITQB/IBET Mass Spectrometry Laboratory performs internal and
external services and related research work. The laboratory installation
process started in October 2002 aiming to be certified as a GLP
unit. At the moment we perform mass determination, structural and
affinity studies for a broad range of chemical compounds, from small
organic and organometalic compounds to peptides, oligosacharides,
nucleotides and proteins. The information obtained with the powerful
mass spectrometry techniques is fundamental for the structural characterization
of chemical and biochemical species. Else than precise mass determination
it is possible to perform controlled fragmentation of the molecular
ions, which allows to get detailed structural information, like
comparative identification of organic compounds, peptide and oligosacharide
sequencing, characterization of post-translational modifications:
these are the so called MS/MS or tandem mass spectrometry experiments.
The mass spectrometry results are important, for example, in monitoring
chemical synthesis, purification and identification of chemical
and biochemical compounds, metabolic and kinetic studies. Instrumentation Thermofinnigan LCQ ion-trap coupled to an HPLC system with the
folowing ion sources: Bruker Esquire 3000plus ion-trap with the folowing ion sources:
Applied Biosystem Voyager STR MALDI-TOF with reflectron * Mass Spectrometry * Small Molecule X-ray Crystallography
Molecular Biology Laboratory
- Institute of Hygiene and Tropical Medicine
The Hepatitis delta virus (HDV) is the causative agent of one of the most severe forms of virus hepatitis, being endemic in several regions of the world. This virus is the smallest human pathogen known so far. It's 1,7 Kb, circular, ss RNA genome – strikingly similar to that of plant viroids -encodes for a single protein that is expressed in two forms as a result of an RNA editing mechanism. Despite being so simple, very little is known about the cellular factors involved in virus replication and biogenesis as well as the pathogenic mechanisms that may cause different disease forms: fulminant hepatitis, cirrhosis, chronic hepatitis. In this project we intend to identify changes in cellular gene expression at the protein level, that arise upon HDV infection, and transient transfection of hepatoma cells with cDNAs encoding the HDV components (RNA, and both forms of antigens). Accomplishment of this goal will hopefully help to shad light on the virus life cycle, and identify novel targets for a specific therapy. Project coordinator: Celso Cunha; Molecular Biology Unit, Institute of Hygiene and Tropical Medicine, Lisbon, Portugal (ccunha@ihmt.unl.pt) Centro de Malária e outras Doenças Tropicais, U.E.I. Malária, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa – Host-parasite interactions
Rua da Junqueira, 96 Missions Malaria is a life threatening disease that kills
over a million each year. The parasite that causes it has a complex
life cycle involving a vertebrate host and a mosquito vector. Most
efforts have been put in controlling malaria in the vertebrate host.
Even so, an efficacious malaria vaccine is far from being achieved
and anti-malarial resistance have been spreading globally. Therefore,
complementary malaria control strategies acting on the vector are
urgently needed. Identification of mechanisms of infection control
and how they can be modulated will permit, in the long run, the
development of transmission blocking strategies. In this frame our
research is focused on the 1) influence of blood meal components
on the outcome of Plasmodium infection in the mosquito; and 2) characterization
of mosquito immune response to Plasmodium infection.
2003-2006 The effect of chloroquine on the modulation of mosquito vector response to Plasmodium infection. ref:POCTI/MGI/344905/2002 2006-2007 Gene variability of pattern-recognition-molecules (PRM) involved in mosquito immune response to Plasmodium. ref: POCI/SAU-IMI/59489/2004 Team Henrique Silveira, Principal Investigador Susana Ramos, PhD Student (FCT*) Luis Filipe Lopes, PhD Student (FCT*) Patrícia Abrantes, PhD Student (FCT*) Rute Felix, PhD Student (FCT*) Bianor Valente, MSc Student * Fundação da Ciência
e a Tecnologia PhD fellowship ProCura-University
Members
Departamento de Química
(DQ/UA)
Since its firsts steps in 1975 of the Mass Spectrometer group at the University of Aveiro the members of our lab are interested in a variety of areas of mass spectrometry. Our focuses include research on the gas phase ion chemistry and biological mass spectrometry. Research projects are from the development of the application of ESI and nano-ESI to Proteomics, Oxidative stress, Biogenic amines and Coordenation compounds and to the fundamental studies of ion energetics. Instrumentation Q-TOF
II mass spectometer with the folowing ion sources:
Mass spectometer AUTOSPEQ (EBEqQ) with the folowing ion sources:
REQUIMTE Laboratório Associado, CQFB, Departamento de Química (FCT/UNL)
Departamento de Química Tel (office): +351 212 948 386; ext. 10907 Missions At Present the main research line of Dr. Capelo
belongs to analytical proteomics and metabolomics. Through drastic
changes introduced in the sample treatments for proteomics using
ultrasonication by Dr. Capelo´s research team, the standard
procedures for protein and peptide identification has been reduced
from days to seconds or few minutes. With the new ultra fast sample
treatments, the identification of virus and bacteria is done in
an easy and rapid manner, allowing rapid medical diagnosis for illnesses
and infections. At present the protocols already published by Dr.
Capelo are being used for the systematic identification of pathogens
(salmonella), in collaboration with some hospitals of the Lisbon
area. Furthermore, the simplification in the methodologies for the
analysis and identification of steroids in blood or urine (analytical
metabolomic) is a priority society´s demand in the fighting
against the fraud in sport. At present, new approaches for the fast
identification of steroids in urine are developed in the research
team of Dr.Capelo. Current Projects Team Dr. Jose Luis Capelo, Researcher Dr. Carlos Lodeiro, Researcher Dr. Joao Paulo Noronha, Researcher Dr. Raquel Rial, Researcher Gonçalo Vale, Undergraduate Student Hugo Santos, MSc student Ricardo Carreira, PhD student Marco Galesio, BSc |
